GeneXpert and Acid-Fast Bacilli Smear for Diagnosis of Pulmonary Tuberculosis in a Tertiary Care Center of Rural Nepal

Introduction: Mycobacterium tuberculosis is the second most infectious cause of death in adults globally and major health trouble Nepal. The diagnosis pulmonary mainly carried by acid-fast bacilli smear microscopy, culture on Lowenstein–Jensen media, GeneXpert MTB/RIF assay. This study aimed to evaluate Acid-Fast Bacilli stain used for tuberculosis.
 Methods: A cross-sectional was performed at Karnali Academy Health Sciences, Jumla, from July 2021 June 2022. Our population comprised 390 probable cases ages 2 92 years. total sputum specimens suspected presumptive sufferers were tested using direct staining technique detection MTB/RIF. compared with smear. Patient’s demographic details, AFB smear, test results collected entered working Performa. Data analyzed SPSS 16.0.
 Results: Out samples 13.84% positive via assay while 5.12% through microscopy which statistically significant (p<.0001). When GeneXpert, taking as gold standard rural setup Nepal sensitivity 37.03%, its specificity 90.81%.
 Conclusions: a rapid that can aid timely tuberculosis, facilitating treatment area.